BRT® (Brilliant Black Reduction Test) is a range of microbiological tests designed to detect inhibitors in milk, particularly antibiotic residues, in cow, sheep, and goat milk. The tests are based on the sensitivity of test bacteria to inhibitory substances, using a colorimetric reduction principle to assess metabolic activity.

The portfolio includes:

  • BRT® Inhibitor Test,
  • BRT® MRL Screening Test,
  • BRT® hi-sense.

The complete system includes the test kits themselves and the required incubation equipment for proper analysis. Categories: Dairy industry, Laboratory tests and equipment

Categories: ,

Features

  • Microbiological test based on the Brilliant Black Reduction principle;
  • Detects antibiotic residues and other inhibitory substances;
  • Applicable to raw and heat-treated milk;
  • Visual interpretation through color change (blue → yellow);
  • Available formats:
    • plates (96 wells),
    • detachable strips,
    • Single 48 (individual tubes);
  • Incubation time:
    • 2h 15 min ± 30 min (Inhibitor & MRL);
    • 3 hr 15 min ± 15 min (hi-sense);
  • Incubate at 65°C.

Advantages

  • Officially recognized method for detecting inhibitors in milk;
  • Sensitive detection of multiple antibiotic classes;
  • Clear result interpretation (negative / suspect / positive / strongly positive);
  • Adaptable for both small laboratories and industrial facilities;
  • Flexible formats depending on testing volume;
  • Good stability under controlled storage conditions.

Applicability

BRT® is used for:

  • control of antibiotic residues in raw milk,
  • milk testing at reception in processing units,
  • monitoring compliance with MRL limits,
  • quality control in farms and collection centers,
  • laboratory analysis in the dairy industry.

Required equipment

For proper use of BRT® tests, the following are required:

  • incubator (thermo-block) or water bath at 65°C,
  • calibrated micropipette for 100 µL (where disposable pipettes are not included),
  • spectrophotometer (optional) for reading at 450 nm / 620 nm.